LIBRARY PREPARATION AND QUANTIFICATION FOR CHROMATIN IMMUNOPRECIPITATION FOLLOWED BY HIGH-THROUGHPUT SEQUENCING (CHIP-SEQ)
DOI:
https://doi.org/10.53555/eijaer.v4i2.34Abstract
Properly generating robust, high-quality libraries for ChIP-Seq will prepare your for Illumina sequencing and an overall successful ChIP-Seq experiment. It is often challenging to prepare DNA for a next-gen ChIP-Seq library because it’s difficult to obtain a large amount of DNA after ChIP. In this paper, the library construction method is suitable for low concentration DNA in woody samples.
References
Adli M, Bernstein BE. Whole-genome chromatin profiling from limited numbers of cells using nano-ChIP-Seq. Nat Protoc. 2011;6(10):1656–68.
Dijk EL, Jaszczyszyn Y, Thermes C. Library preparation methods for next-generation sequ encing: tone down the bias.[J]. Experimental Cell Research, 2014, 322(1):12-20.
M.L. Metzker,Sequencingtechnologies–the nextgeneration, Nat. Rev.Genet.11(2010)31–46.
Shankaranarayanan P, Mendoza-Parra MA, Walia M, Wang L, Li N, Trindade LM, Grone meyer H. Single-tube linear DNA amplification (LinDA) for robust ChIP-Seq. Nat Methods. 2 011;8(7):565–7.
Wang Z, Gerstein M, Snyder M. RNA-Seq: a revolutionary tool for transcriptomics[J]. Nature Reviews Genetics, 2009, 10(1):57-63.
Downloads
Published
Issue
Section
License
Copyright (c) 2018 EPH - International Journal of Agriculture and Environmental Research
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.